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Our CRISPR transgenic strains were published in Developmemtal Cell

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2018-10-29 15:40:55


It is definitely a good news that SunyBiotech's CRISPR transgenic C. elegans are quoted by world renowned academic journal!

 

SunyBiotech is a professional company specializing in providing gene editing service in C. elegans using the CRISPR/Cas9 system.All strains generated by us should be named under Sunybiotech’s nomenclature. PHX is the strain designation and syb is the allele designation. Recently,one of our CRISPR transgenic strains was published on Developmental Cell, the impact factor of which is 9.174. Another strain was cited in BioRxiv, the preprint server for biology, operated by Cold Spring Harbor Laboratory.

 

1. Channel Nucleoporins Recruit PLK-1 to Nuclear PoreComplexes to Direct Nuclear Envelope Breakdown in C. elegans [1]


Figure 1. Schematic diagrams showing PLK-1 dynamics and targetingmechanisms during prophase [1]

 

This research was achieved by Dr. Lionel Pintard’s team, which mainly discovered how mitotic kinases trigger the Nuclear EnvelopeBreakdown (NEBD) in C. elegans.

 

In this research, npp-1(syb207) mutant was generated by SunyBiotech. The syb207 allele is an in-frame deletion, in which 1604bp coding sequence (from 19thbpto 1622ndbp) is deleted. This mutant was used to study the recruitmentof plk-1 and its effect in the NEBD.

 

2.     Thechromatin modifiers SET-25 and SET-32 are required for initiation but not longterm  maintenance of transgenerationalepigenetic inheritance [2]

Figure 2 theexpression pattern of set-32::mcherry knock-in strain [2]

 

The research was accomplished by Dr. Alyson Ashe's group, which mainly discovered the function of histone methyl transferases SET-25 and SET-32 during the transgenerational epigenetic inheritance.

 

During the research, the Alyson Ashe's team ordered the PHX229 set-32(syb229) worms fromSunybiotech. This knock-in was generated by inserting the mCherry coding sequence at the 3' terminal of the set-32 gene, which was used to study the expression position of the set-32 protein (Figure 2).

 

You are invited to tell us when you figure out the syb alleles while reading papers. Sunybiotech has generated about 600 CRIPSR transgenic strains by now.We will try our best to provide more professional and efficient C.elegans editing service in the future.

 

Reference:

[1]MartinoL, Morchoisne-Bolhy S, Cheerambathur DK, VanHove L, DumontJ, JolyN, DesaiA, DoyeV, Pintard L. Channel Nucleoporins Recruit PLK-1to Nuclear Pore Complexes to Direct Nuclear Envelope Breakdown in C. elegans. Dev Cell. 2017, 43(2):157-171.  

[2] Woodhouse R, Buchmann G, HoeM, Harney D, Larance M, Boag PR, Ashe A,The chromatin modifiers SET-25and SET-32 are required for initiation but not long-term maintenance oftransgenerational epigenetic inheritance.bioRxiv preprint first posted onlineJan. 29, 2018;doi: https://doi.org/10.1101/255646


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