Precise Gene Editing

Gene Editing Services via the CRISPR/Cas9 System:

We have established an efficient genome editing system in C. elegans via the CRISPR/Cas9 system (Figure 1) and can generate strains with desired sequence changes quickly (Figure 2). These include precise gene deletion, precise nucleotide substitutions, precise sequence knock-in, and precise sequence replacement.


   Figure 1 Schematic diagram of gene editing via the CRISPR/Cas9 system

    Figure 2 Categories of CRISPR/Cas9 gene editing services


   Procedures and Price of Services:

Table 1 Procedures of CRISPR/Cas9 gene editing services
Editing    reagent PreparationsgRNA plasmids and    oligonucleotide or plasmid templatesHigh quality plasmids and    templates
MicroinjectionThe C.elegans strain designated10-30 adults
ScreeningF1 and/or F2 progeny of injected wormsAnimals with the desired editing
Final    verificationAnimals containing the desired sequence changesVerification by sequencing
Table    2 Price of CRISPR/Cas9 gene editing services
Gene editing via the CRISPR/Cas9    systemTurnaround(weeks)Price(US$)/strain
A002Precise sequence/gene deletion(≤1500bp)**6-8$899(1st)*/ $458(2nd)/ $258(3rd)
A003Precise nucleotide changes6-8$899(1st)*/ $458(2nd)/ $258(3rd)

Precise sequence knock-in


6-8$1399(1st)*/ $699(2nd)/ $458(3rd)

Precise sequence replacement


6-8$1499(1st)*/ $749(2nd)/ $499(3rd)
 *: 1st, 2nd, 3rd means 1st, 2nd or 3rd parallel lines obtained in one injection, irradiation or gene editing.
**: Please contact us for quote if the edited sequence size is larger than the number in the price list.


   1:Edited strains verified by sequencing
   2:Sequencing Report

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