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Precise Gene Editing

Gene Editing Services via the CRISPR/Cas9 System:

We have established an efficient genome editing system in C. elegans via the CRISPR/Cas9 system (Figure 1) and can generate strains with desired sequence changes quickly (Figure 2). These include random gene insertion/ deletion, precise gene knock-out, precise nucleotide substitutions, precise sequence knock-in, and precise small tag knock-in.

 


   Figure 1 Schematic diagram of gene editing via the CRISPR/Cas9 system


    Figure 2 Categories of CRISPR/Cas9 gene editing services

 

   Procedures and Price of Services:


Table 1 Procedures of CRISPR/Cas9 gene editing services
ProceduresMaterialsStandard
Editing    reagent PreparationsgRNA plasmids and    oligonucleotide or plasmid templatesHigh quality plasmids and    templates
MicroinjectionThe C.elegans strain designated10-30 adults
ScreeningF1 and/or F2 progeny of injected wormsAnimals with the desired editing
Final    verificationAnimals containing the desired sequence changesVerification by sequencing
Table    2 Price of CRISPR/Cas9 gene editing services
PID
Gene editing via the CRISPR/Cas9    systemTurnaround(weeks)Price(US$)/strain
A002Precise sequence/gene deletion(≤1500bp)**6-8$899(1st)*/ $458(2nd)/ $258(3rd)
A003Precise nucleotide changes6-8$899(1st)*/ $458(2nd)/ $258(3rd)
A004Precise sequence knock-in(GFP,mCherry,etc.)6-8$1399(1st)*/ $699(2nd)/ $458(3rd)
A005Precise small-tag knock-in(Flag,HA tag,etc.)6-8$1399(1st)*/ $699(2nd)/ $458(3rd)
 *: 1st, 2nd, 3rd means 1st, 2nd or 3rd parallel lines obtained in one injection, irradiation or gene editing.
**: Please contact us for quote if the precise sequence deletion is larger than 1500bp or the knock-in sequence is larger than 900bp.



 

Deliverables:
   1:Edited strains verified by sequencing
   2:Sequencing Report


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