Gene Editing Services via the CRISPR/Cas9 System:
We have established an efficient genome editing system in C. elegans via the CRISPR/Cas9 system (Figure 1) and can generate strains with desired sequence changes quickly (Figure 2). These include random gene insertion/ deletion, precise gene knock-out, precise nucleotide substitutions, precise sequence knock-in, and precise small tag knock-in.
Figure 1 Schematic diagram of gene editing via the CRISPR/Cas9 system
Figure 2 Categories of CRISPR/Cas9 gene editing services
Procedures and Price of Services：
|Table 1 Procedures of CRISPR/Cas9 gene editing services|
|Editing reagent Preparation||sgRNA plasmids and oligonucleotide or plasmid templates||High quality plasmids and templates|
|Microinjection||The C.elegans strain designated||10-30 adults|
|Screening||F1 and/or F2 progeny of injected worms||Animals with the desired editing|
|Final verification||Animals containing the desired sequence changes||Verification by sequencing|
|Table 2 Price of CRISPR/Cas9 gene editing services|
|PID||Gene editing via the CRISPR/Cas9 system||Turnaround(weeks)||Price(US$)/strain|
|A002||Precise sequence/gene deletion(≤1500bp)**||6-8||$899(1st)*/ $458(2nd)/ $258(3rd)|
|A003||Precise nucleotide changes||6-8||$899(1st)*/ $458(2nd)/ $258(3rd)|
|A004||Precise sequence knock-in(GFP,mCherry,etc.)||6-8||$1399(1st)*/ $699(2nd)/ $458(3rd)|
|A005||Precise small-tag knock-in(Flag,HA tag,etc.)||6-8||$1399(1st)*/ $699(2nd)/ $458(3rd)|
|*: 1st, 2nd, 3rd means 1st, 2nd or 3rd parallel lines obtained in one injection, irradiation or gene editing.|
|**: Please contact us for quote if the precise sequence deletion is larger than 1500bp or the knock-in sequence is larger than 900bp.|
1：Edited strains verified by sequencing
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