Gene Editing Services via the CRISPR/Cas9 System:
We have established an efficient genome editing system in C. elegans via the CRISPR/Cas9 system (Figure 1) and can generate strains with desired sequence changes quickly (Figure 2). These include random gene insertion/ deletion, precise gene knock-out, precise nucleotide substitutions, precise sequence knock-in, and precise small tag knock-in.
Figure 1 Schematic diagram of gene editing via the CRISPR/Cas9 system
Figure 2 Categories of CRISPR/Cas9 gene editing services
Procedures and Price of Services：
|Table 1 Procedures of CRISPR/Cas9 gene editing services|
|Editing reagent Preparation||sgRNA plasmids and oligonucleotide or plasmid templates||High quality plasmids and templates|
|Microinjection||The C.elegans strain designated||10-30 adults|
|Screening||F1 and/or F2 progeny of injected worms||Animals with the desired editing|
|Final verification||Animals containing the desired sequence changes||Verification by sequencing|
|Table 2 Price of CRISPR/Cas9 gene editing services|
|PID||Gene editing via the CRISPR/Cas9 system||Turnaround(weeks)||Price(US$)/strain|
|A002||Precise sequence/gene deletion(≤1500bp)**||6-8||$899(1st)*/$458(2nd)/$258(3rd)|
|A003||Precise nucleotide changes||6-8||$899(1st)*/$458(2nd)/$258(3rd)|
|A004||Precise sequence knock-in(GFP,mCherry,etc.)||6-8||$1399(1st)*/$999(2nd)/$599(3rd)|
|A005||Precise small-tag knock-in(Flag,HA tag,etc.)||6-8||$1399(1st)*/$999(2nd)/$599(3rd)|
|*: 1st, 2nd, 3rd means 1st, 2nd or 3rd parallel lines obtained in one injection, irradiation or gene editing.|
|**: Please contact us for quote if the random/precise sequence deletion is larger than 1500bp|
1：Edited strains verified by sequencing
How to Order:
To get a quote or place an order, please register and login your account, download and fill out the order form(s) of the service(s) you need, and email the form(s) to firstname.lastname@example.org. We will send the confirmed order form with quote to your email address (the one related to your account) after checking the information.
Have Other Questions?
You can always reach us at email@example.com. Our customer service and editing experts will get back to you ASAP.