Traditional Transgenic Services:
Traditional transgenic service will be performed by microinjection of the DNA mixture into C. elegans gonads to generate extrachromosomal transgenic arrays that contain high copy numbers of the injected DNA constructs (Figure 1).
When animals carrying extrachromosomal transgenic arrays are exposed to X ray, γ ray or ultraviolet (UV) irradiation, chromosome breaks are induced, and during the process of DNA repair, the extrachromosomal transgenic arrays will be inserted into the chromosomes to form integrated transgenic arrays.
Procedures and Price of Services：
|Table.1 Procedure of traditional transgenic service|
|Extrachromosomal arrays||Sample preparation||Plasmids of interest and co-injection marker||High quality plasmids prepared by Qiagen Mini Plasmid Kit||14-21 days|
|Microinjection||The nematode strain designated||10-30 adults injected|
|Screen||F2 progeny of injected worms||F2 animals with stable extrechromosomal arrays|
|Integrated transgene arrays||γ ray irradiation||Animals with stable extrechromosomal arrays||35-42 days|
|Screen||F3-F5 progeny after irradiation||Integrated lines|
|Delivery||Integrated transgenic lines||Every progeny should carry the integrated transgene based on the co-injection marker|
|Table.2 Price of traditional transgenic service|
|PID||Traditional transgenic nematodes||Turnaround(weeks)||Price(US$)/Strain|
|B002||Integrated transgenic array||8-10||$699(1st)*/$359(2nd)/$258(3rd)|
|*:1st, 2nd, 3rd means 1st, 2nd or 3rd parallel lines obtained in one injection, irradiation or gene editing.|
|**: Please contact us for quote if the random/precise sequence deletion is larger than 1500bp|
Transgenic strains with extrachromosomal arrays or integrated transgenic arrays containing constructs of interest.
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