Oxidative Stress Analysis Services

Oxidative stress, caused by the production of reactive oxygen species (ROS) and reactive nitrogen species under harmful internal and external stimuli, plays a key role in cellular and tissue dysfunction. C. elegans serves as an excellent model organism for studying antioxidant functions due to its similarity to the human antioxidant system, including comparable antioxidant enzymes. By assessing ROS level and monitoring the lifespan of C. elegans, researchers can explore drugs and compounds mechanisms, analyze gene regulatory network and better understand oxidative stress-related pathways.

Our Services include but are not limited to:

ØOxidative Stress Model Construction

Develop C. elegans oxidative stress models using compounds like paraquat and juglone to induce stress condition.

ØAntioxidant Drug Evaluation

The effects of antioxidant drugs were evaluated by observing the survival of C. elegans after oxidative stress, detecting the changes in the content of antioxidant enzymes (SOD, CAT, etc.), and measuring the level of ROS in C. elegans using H2DCFDA probe.

Fig.1 ROS phenotype of Caenorhabditis elegans after H2DCFDA staining

Fig. 2 Average fluorescence intensity of ROS in Caenorhabditis elegans

Related Services

H007-Lifespan assay

H009-Oxidative stress assay

H013-ROS measurement assay

H014-SOD activity assay

H015-CAT activity assay

C002-Quantitative Real-time PCR

D002-Transcriptome sequencing