If the gene deletion is found non-homozygosable, the heterozygote is sequenced and becomes the deliverable. If you want balanced heterozygotes, please contact us to order the Balancer Cross Service.
Worms we send out has strain name and allele name labelled on the agar plates. We will also send the strain name and genotype to you in the sequence summary file. PHX is our strain designation and syb is our allele designation. Both PHX and syb are confirmed by the WormBase. Please use our allele name in your publications.
If you are requesting gene editing service(s), you do not need to provide the plasmid as we will make sgRNA plasmid and donor ourselves. But if your knock-in sequence is specific and we don’t have the template, please send the template plasmid to us. If you are requesting B001 and B002 service, you can either send us the plasmid or request us to construct one (C001 Service) for you.
To get a quote or place an order, you can choose either ways:
1) register and login your account, download and fill out the order form(s) of the service(s), and email the form(s) to service@sunybiotech.com ;
2) reach us at service@sunybiotech.com, we will send you the order forms.
1) Target plasmid
2) Report markers
3) Background strain on which you want to do microinjection.
Please make the payment within 7 days since you have received the package and verified that the engineered strains are workable.
1) Gene name
2) The mutation you want to generate or the sequence you want to delete or insert.
3) Strain on which you want to do gene editing.
If it is your first time placing an order with us and the total amount is no more than 3000 USD, no pre-payment is required. Otherwise, we will require a 50% pre-payment prior to providing the service.
We accept wire transfer and credit card payment.
No. The prices listed in the pricing table are service charges only. Tax/duties, shipping and handling fee are not included. When you proceed to purchase your order, sales tax and shipping & handling fee will be calculated on the total value and shipping charges, and displayed on the invoice we email to you.
We don't charge for the failure of generating strains due to our fault. If you have made the pre-payment, we will return the pre-payment to you.
We usually ship the packages out via DHL / FedEx which normally takes no more than 5 days to arrive.
You are able to check the order status in our website (http://www.sunybiotech.com).
What you need is to click the menu "Log in" on the front page and input "User name" and "Password". For the first time login, please contact us for account details.
Once you get into the account, you can click "My Order" button to check the progress of all your orders.
We will email you the tracking number after we have shipped your package.
We will send the strain again if the client found the strain dead upon delivery. Please report the status within 15 days, otherwise, we will consider that the strain is all right.
For any problems of our strains, please don’t hesitate to contact us in your earliset convenience, our technicians will provide professional resolutions to the problem. We will try our best to satisfy you.
SunyBiotech will perform sequencing to the edited worms, and the sequencing area covers about 300bp on either side of the editing site. We will email you the sequencing report when we send the worms out to you.