Traditional C. elegans Transgenic services
Traditional transgenic services include generating extrachromosomal arrays and integrated arrays. These services will generate transgenic animals with multiple-copy transgenes, and result in over expression of the target plasmid.
These services begin from the microinjection of the DNA mixture into C. elegans gonads to generate extrachromosomal transgenic arrays that contain high copy numbers of the injected DNA constructs. For the X-ray mediated integrated arrays, the extrachromosomal arrays will be treated with X-ray irradiation, and screened for integrated arrays. The integration site is random and could be determined by mapping.
Figure 1 Schematic diagram of generating extrachromosomal arrays and X-ray induced integrated arrays
Plasmid Construction
Vector modifications (replacement of promoter, coding sequence or 3' UTR sequence), sub-cloning and site-directed mutagenesis are common techniques used in molecular biology experiments. SunyBiotech can provide efficient vector modification, sub-cloning, and site-directed mutagenesis service based on customers' needs. Moreover, we can do vector modifications using a ligation-independent method. Therefore, no additional restriction enzyme sites or linker sequences will be added.
| PID | Service | You need to provide | Turnaround(wk) | Price(US$) | Deliverables |
|---|---|---|---|---|---|
| C001 | Plasmid Construction | 1. Information of the vector backbone 2. The modification you need | 2-3 | Starts from 200 USD* | Plasmid and sequencing reports |
| B001 | Extra-chromosomal arrays |
| 3-4 | 500 USD（≥3 lines） | Extrachromosomal lines that contain the target plasmid and report markers |
| B002 | X-ray mediated integrated arrays | 8-10 | 1049 USD（1 line） | Integrated lines that contain the target plasmids and report markers |
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