CRISPR/Cas9 Bacterial Gene Editing Service

Thanks to the convenient CRISPR/Cas9 gene editing system, gene editing in species such as yeast, nematodes, drosophila, zebrafish, mice, and human cells has become more efficient. Currently, we have established a complete CRISPR/Cas9-mediated bacterial gene editing system (Figure 1) that can quickly and efficiently complete the following gene editing: precise sequence knockout, precise single nucleotide mutation, precise sequence insertion, precise short protein tag insertion, and precise sequence replacement.

Figure 1    CRISPR/Cas9 Gene Editing System

As a classic model organism for life science research, bacteria have wide applications in both basic and applied research.

In basic research:

1. 1.Genomics research: E. coli is an ideal model for studying fundamental life processes, such as gene expression, DNA replication, transcription, and translation.
2. 2.Genetics research: Bacteria have a simple genetic background, making gene mutation, screening, and localization easily.
3. 3.Medical research: Mechanism research of how gut microbiota and their metabolites affect metabolism, health, and disease.

In applied research:

1. 1.Recombinant protein expression: Using genome modified bacteria to produce various recombinant proteins, such as insulin, interferon, vaccine antigens, etc.
2. 2.Metabolic engineering and synthetic biology: Modifying bacterial metabolic pathways to produce and synthesize industrial chemicals and biofuels, such as lactic acid, pigment ethanol, butanol, fatty acids, etc.
3. 3. Environmental remediation and biopesticides: Creating genome edited beneficial bacteria for agriculture and fisheries, which can degrade pesticide residues, treat pests and diseases, or pathogens, such as Nocardia, Bacillus thuringiensis, etc.
4. 4. Pharmaceutical development: Studying the pathogenic mechanisms of pathogenic bacteria or genetically modified pathogenic bacteria to help develop new antibiotics and treatments, such as Salmonella, Mycobacterium tuberculosis, etc.
5. 5. Drug screening and toxicology testing: Using genetically modified pathogenic bacteria to rapidly and cost-effectively screen drugs and conduct toxicology tests.

Table 1 Service Process for Bacterial Transgenic Technology

Table 2. Prices for Bacterial Gene Editing Services

Deliverables:

1. 1.Edited strains verified by sequencing
2. 2.Sequencing report

How to Order:

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FAQs

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